Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney

FEBS Letters

Bagnasco, Serena¹  Barone, Virginia²  Galietta, Luis J.V.²  Romeo, Giovanni²  Rasola, Andrea² 

[1] Laboratorio di Anatomia e Istologia Patologica, Istituto G. Gaslini, L.go G. Gaslini, 16148 Genova, Italy;Laboratorio di Genetica Molecolare, Istituto G. Gaslini, L.go G. Gaslini, 16148 Genova, Italy

关键词: GABA;    Betaine;    Kidney;    Transporter;    Cell volume;    Chromosomal localization;   

DOI  :  10.1016/0014-5793(95)01052-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a K _m = 20 μM for GABA transport and a coupling to Na⁺ and Cl⁻ with a stoichiometry 3 Na⁺:2 Cl⁻:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.

【 授权许可】

Unknown