Modulation of tumor necrosis factor‐α release by anisoosmolarity and betaine in rat liver macrophages (Kupffer cells)

FEBS Letters

Häussinger, Dieter¹  Warskulat, Ulrich¹  Zhang, Fan¹ 

[1]Medizinische Universitätsklinik, Klinik für Gastroenterologie, Hepatologie und Infektiologie, Heinrich Heine University, Moorenstrasse 5, D-40225 Düsseldorf, Germany

关键词: Kupffer cell;    Betaine;    Osmolyte;    Hyperosmolarity;    Cell volume;    Osmolarity;    Tumor necrosis factor;    Macrophage;    BGT-1;    Anti-rMu-TNF-α mAb;    anti-mouse recombinant tumor necrosis factor-α monoclonal antibody;    Cox-1;    cyclooxygenase-1 (constitutive);    Cox-2;    cyclooxygenase-2 (inducible);    LPS;    lipopolysaccharide;    PGE2;    prostaglandin E2;    FCS;    fetal calf serum;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    TNF-α;    tumor necrosis factor-α;   

DOI  :  10.1016/0014-5793(96)00754-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hypoosmotic exposure (205 mosmol/l) of rat liver macrophages together with lipopolysaccharide (LPS) inhibited the LPS-induced tumor necrosis factor-α (TNF-α) release by about 60% and markedly diminished the LPS-induced increase of TNF-α mRNA levels. Hyperosmotic exposure (405 mosmol/l) had no effect on total TNF-α release, however, both TNF-α accumulation in the medium and the LPS-induced increase of TNF-α mRNA levels were significantly delayed under these conditions. This delay was abolished upon addition of betaine, which acts as an osmolyte in Kupffer cells. When LPS was added to Kupffer cells that had been preexposed to hyperosmotic medium for 24 h, the LPS-induced TNF-α release was inhibited by 90% when compared to normoosmotic conditions. Likewise, the LPS-induced increase in TNF-α mRNA levels was largely abolished. Inhibition of TNF-α release and of the increase in the TNF-α mRNA level in response to hyperosmolarity/LPS, however, was largely overcome when indomethacin or betaine was present during the hyperosmotic preincubation period. Because betaine has recently been shown to inhibit the hyperosmolarity-induced induction of cyclooxygenase-2 and stimulation of prostaglandin production, these findings suggest that the effect of betaine in restoring the LPS-induced TNF-α response in hyperosmotically exposed Kupffer cells is mediated by an inhibition of prostaglandin synthesis. The findings point to a regulatory role of cell volume and betaine for TNF-α production by liver macrophages, suggesting a new role of osmolytes in modulating immune function.

【 授权许可】

Unknown