Molecular cloning of an orphan transporter A new member of the neurotransmitter transporter family

FEBS Letters

Borden, Laurence A.¹  Fried, Steven G.¹  Gustafson, Eric L.¹  Durkin, Margaret M.¹  Weinshank, Richard L.¹  Branchek, Theresa A.¹  Smith, Kelli E.¹ 

[1] Synaptic Pharmaceutical Corporation, 215 College Road, Paramus, NJ 07652, USA

关键词: Transporter;    mRNA localization;    Molecular cloning;    Astrocyte;    Brain (rat);    GABA;    γ-aminobutyric acid;    CSF;    cerebrospinal fluid;    TM;    transmembrane domain;    RT-PCR;    reverse transcriptase-polymerase chain reaction;   

DOI  :  10.1016/0014-5793(94)01328-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A complementary DNA clone predicted to encode a novel transporter was isolated from rat brain and the localization of its mRNA was examined. The cDNA, designated rB21a, predicts a protein with 12 putative transmembrane domains that exhibits significant sequence homology with neurotransmitter transporters. Expression studies have not yet identified the endogenous substrate for this transporter, but the presence of rB21a mRNA within the leptomeninges of the brain suggests the transporter may regulate CSF levels of its substrate. The cloning of rB21a provides the means to determine its physiological functions and the potential to design novel, transporter-based therapeutic agents for neurological and psychiatric disorders.

【 授权许可】

Unknown