Expression of the cyclin‐dependent kinase inhibitor p16 during the ongoing cell cycle

FEBS Letters

Soucek, Thomas¹  Bernaschek, Gerhard¹  Hengstschläger-Ottnad, Elke²  Wawra, Edgar³  Pusch, Oliver¹  Hengstschläger, Markus¹ 

[1] Obstetrics and Gynecology, University of Vienna, Department of Prenatal Diagnosis and Therapy, Währinger Gürtel 18-20, A-1090 Vienna, Austria;Institute of Molecular Genetics, Vienna Biocenter, University of Vienna, Dr. Bohrgasse 9, A-1030 Vienna, Austria;Institute of Molecular Biology, Vienna Biocenter, University of Vienna, Dr. Bohrgasse 9, A-1030 Vienna, Austria

关键词: p16;    Cell cycle;    Centrifugal elutriation;    Cyclin D1;    pRb;   

DOI  :  10.1016/0014-5793(95)01033-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been demonstrated that protein expression of p16, the inhibitor of cyclin-dependent kinase 4 and 6, increases 4 fold at the G1/S transition when serum-arrested cells are restimulated to logarithmic growth. We examined the cell cycle regulation of this cyclin-dependent kinase inhibitor in cells separated according to their cell cycle phases by centrifugal elutriation. Neither p16 mRNA nor its protein expression are regulated during the cell cycle of normal phytohemagglutinin-stimulated lymphocytes, retinoblastoma protein-negative cells, papilloma virus-transformed cells, and acute promyelocytic leukemia cells. p16 mRNA is constitutively expressed in cells in which we detected the normal E2F-dependent S-phase specific expression of thymidine kinase mRNA. We further observed a G1-phase specific expression of cyclin D1 mRNA in the same cells separated by centrifugal elutriation.

【 授权许可】

Unknown