FEBS Letters | |
Biochemical complementation studies in vitro of gyrase subunits from different species | |
Roth, Martin1  Simon, Hannelore2  Zimmer, Christoph2  | |
[1] Hans-Knöll-Institut für Naturstoff-Forschung, e. V., Beutenbergstraβe 11, D-07745 Jena, Germany;Institut für Molekularbiologie, Friedrich-Schiller-Universität Jena, Winzerlaer Straβe 10, D-07745 Jena, Germany | |
关键词: DNA gyrase; Subunit; Gyrase hybrid; DNA binding; DNA cleavage; Immunology; | |
DOI : 10.1016/0014-5793(95)01007-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To investigate the functional equivalence of DNA gyrase subunits from different bacterial sources hybrid enzymes were formed using purified A and B subunits from three species of Streptomycetes, E. coli and B. subtilis. The activity of gyrase hybrids composed of heterologous gyr A and gyr B proteins and of the gyrases containing homologous subunits was characterized by binding studies and a cleavage assay with two different DNA fragments. Likewise the enzyme activity was monitored by the super-coiling and relaxation assay with pBR322 DNA. We found that cleavage reactions are largely determined by the source of the gyr B subunits whereas DNA supercoiling and relaxation reactions of pBR322 catalyzed by DNA gyrase are limited to a combination of homologous A and B subunits or of heterologous A and B subunits from the taxonomically related bacteria Streptomycetes.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020301707ZK.pdf | 433KB | download |