FEBS Letters | |
Regulation of DNA supercoiling in Escherichia coli: Genetic basis of a compensatory mutation in DNA gyrase | |
Fisher, L.Mark1  McEachern, Fiona1  | |
[1] Department of Cellular and Molecular Sciences, Division of Biochemistry, St George's Hospital Medical School, University of London, England | |
关键词: DNA supercoiling; DNA gyrase; DNA topoisomerase I; Compensatory mutation; (Escherichia coli); | |
DOI : 10.1016/0014-5793(89)80931-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a topA deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold, and thereby redressing the intracellular level of supercoiling. The mutant gene differs from the wild type in carrying three rather than two direct tandem repeats of a 6 bp sequence encoding Ala-Arg. We suggest this novel mutation affects domain spacing and was generated by an unequal crossing over event, possibly involving gyrase.
【 授权许可】
Unknown
【 预 览 】
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