Expression of the nir and nor genes for denitrification of Pseudomonas aeruginosa requires a novel CRP/FNR‐related transcriptional regulator, DNR, in addition to ANR

FEBS Letters

Igarashi, Yasuo¹  Arai, Hiroyuki¹  Kodama, Tohru¹ 

[1] Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bankyo-ku, Tokyo 113, Japan

关键词: Denitrification;    Nitrite reductase;    Nitric oxide reductase;    FNR;    ANR;    Pseudomonas aeruginosa;   

DOI  :  10.1016/0014-5793(95)00885-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene, designated dnr, was identified in the vicinity of the structural genes for nitrite reductase (nirS) and nitric oxide reductase (norCB), and the gene for activation of the reductases (nirQ) from Pseudomonas aeruginosa. It encodes a protein of 227 amino acids homologous with the CRP/FNR-family transcriptional regulators. Promoter activities for nirS, nirQ and norCB were considerably reduced in the dnr mutant as well as in the mutant of anr, the other fnr-like regulatory gene from P. aeruginosa. This is the first finding that two CRP/FNR-related regulators are involved in denitrification in one strain.

【 授权许可】

Unknown