Interdependence of respiratory NO reduction and nitrite reduction revealed by mutagenesis of nirQ, a novel gene in the denitrification gene cluster of Pseudomonas stutzeri

FEBS Letters

Jüngst, Angelika¹  Zumft, Walter G.¹ 

[1] Lehrstuhl für Mikrobiologie, Universität Karlsruhe, Kaiserstrasse 12, W-7500 Karlsruhe 1, Germany

关键词: Nitrite respiration;    Cytochrome cd 1;    Nitric oxide reductase;    Denitrification;    NtrC family;    Nucleotide sequence;    Pseudomonas stutzeri;    Pseudomonas aeruginosa;   

DOI  :  10.1016/0014-5793(92)81495-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An open reading frame, designated nirQ, was identified upstream or nirS, the structural gene for the respiratory nitrite reductase or Pseudomonas stutzeri ZoBell. Its derived gene product (275 amino acids, M _r = 30.544) shows similarity to the NtrC protein family or transcriptional activators. Deletion—replacement mutagenesis of the nirQ gene resulted in the simultaneous loss of nitrite reduction and NO reduction in vivo. However, both reductases were still synthesized, with only nitrite reductase being active in vitro. NO reductase was overproduced by a factor of about 2. Our results indicate that the systems for nitrite reduction and NO reduction are functionally coupled.

【 授权许可】

Unknown