The nirSTBM region coding for cytochrome cd 1‐dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono‐, di‐, and tetraheme proteins

FEBS Letters

Jüngst, Angelika¹  Wakabayashi, Sadao²  Matsubara, Hiroshi²  Zumft, Walter G.¹ 

[1] Lehrstuhl für Mikrobiologie, Universität Karlsruhe, 12, D-7500 Karlsruhe 1, Germany;Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan

关键词: Nitrite respiration;    Cytochrome cd 1;    Cytochrome c 551;    Cytochrome c 552;    Tetraheme protein;    Signal peptide;    Pseudomonas stutzeri;    ORF(s);    open reading frame(s);    PAGE;    polyacrylamide gel electrophoresis;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/0014-5793(91)80150-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Genes for respiratory nitrite reduction (denitrification) of Pseudomonas stutzeri are clustered within 7 kbp. A 4.6-kbp Hind III-Kpn I fragment carrying nirS, the structural gene for cytochrome cd ₁, was sequenced. An open reading frame immediately downstream of nirScodes for a 22.8-kDa protein with four heme c-binding motifs. Mutagenesis of this gene causes an apparent defect in electron donation to cytochrome cd ₁. Following this ORF are the structural genes for cytochrome c ₅₅₂, cytochrome c ₅₅₁, and ORF5 that codes for a 11.9-kDa monoheme protein. All cytochromes have a signal sequence for protein export.

【 授权许可】

Unknown