Long‐term enhancement of Na,K‐ATPase pump during blasttransformation of human lymphocytes is controlled first by translational, then by transcriptional mechanisms

FEBS Letters

Marakhova, I.I.¹  Vinogradova, T.A.¹  Toropova, F.V.¹  Vereninov, A.A.¹ 

[1] Institute of Cytology, Academy of Sciences, 194064 St. Petersburg, Russian Federation

关键词: Lymphocyte activation;    Na;    K-ATPase pump;    Cyclosporin A;    Cycloheximide;    Actinomycin D;    α-Amanitin;   

DOI  :  10.1016/0014-5793(95)00608-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transition of phytohemagglutinin-activated human lymphocytes from resting state to proliferation is accompanied by a long-term increase in ouabain-sensitive Rb(K) influx which is closely related to a cyclosporin A-sensitive step of G0/G1/S progression. At least two distinct phases of the up-regulation of cation pump has been revealed: the initial stage (5–20 h) which is cycloheximide-inhibitable and actinomycin D (α-amanitin)-unaffected, and the later stage (after 20 h) which is cycloheximide- and actinomycin D (α-amanitin)-inhibitable. Thus, the enhanced Na,K-ATPase pump during the cell progression from quiescence to proliferation is controlled both at translational and transcriptional levels.

【 授权许可】

Unknown