Transcriptional regulation of carbonic anhydrase II by retinoic acid in the human pacreatic tumor cell line DANG

FEBS Letters

Riecken, Ernst-Otto¹  Rosewicz, Stefan¹  Stier, Ute¹ 

[1] Department of Gastroenterology, Medizinische Klinik und Poliklinik, Klinikum Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany

关键词: Carbonic anhydrase II;    Retinoic acid;    Pancreas;    DANG cell;   

DOI  :  10.1016/0014-5793(95)00596-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carbonic anhydrase II (CA II) generates bicarbonate in human pancreatic duct cells. We have developed the human pancreatic duct cell line DANG as a model to study the effects of all-trans-retinoic acid (ATRA) on CA II gene expression. ATRA treatment resulted in a time- and dose-dependent inhibition of CA II mRNA concentrations in DANG cells. These inhibitory effects were paralleled by a time-dependent decrease of CA II protein concentrations. Nuclear run on analysis revealed that the decrease of CA II mRNA concentrations was due to a decreased rate of CA II gene transcription. These data show that ATRA transcriptionally modulates CA II gene expression in human pancreatic carcinoma cells.

【 授权许可】

Unknown