Fra‐1: a novel target for retinoid action

FEBS Letters

Kaiser, Astrid¹  Riecken, Ernst-Otto¹  v. Marschall, Zofia¹  Wiedenmann, Bertram²  Rosewicz, Stefan¹  Brembeck, Felix H.¹ 

[1] Medizinische Klinik mit Schwerpunkt Gastroenterologie/Hepatologie, Department of Gastroenterology, Klinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany;Dept. of Hepatology/Gastroenterology, Universitätsklinikum Charité, Campus Virchow-Klinikum, Humboldt Universität, Augustenburgerplatz 1, 13353 Berlin, Germany

关键词: Fra-1;    Retinoic acid;    Pancreas;    Activator protein 1;   

DOI  :  10.1016/S0014-5793(99)00326-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a cDNA gene expression array system, we identified fra-1 as a novel target molecule for retinoid action in the human pancreatic carcinoma cell line Dan-G. Retinoid treatment resulted in a significant and time-dependent induction of the fra-1 expression on the post-transcriptional level. Supershift assays revealed that fra-1 participates in the activator protein 1 complex together with c-fos, c-jun and junB. Transient transfection experiments using a reporter plasmid containing an activator protein site upstream of the CAT reporter gene revealed that the phorbolester-induced CAT activity was suppressed by retinoids. Since fra-1 lacks a transactivation function, we therefore suggest that the retinoid-mediated induction of fra-1 might function as a negative regulator of the activator protein 1 activity in human pancreatic carcinoma cells.

【 授权许可】

Unknown