Carbonic anhydrase II is induced in HL‐60 cells by 1,25‐dihydroxyvitamin D3: A model for osteoclast gene regulation

FEBS Letters

Shapiro, Linda Hillstrom¹  Yu, Ya-Shiou L.¹  Tashian, Richard E.¹  Venta, Patrick J.¹ 

[1] Department of Human Genetics, University of Michigan, Medical School, Ann Arbor, MI 48109-0618, USA

关键词: Carbonic anhydrase II;    Osteoclast;    Dihydroxyvitamin D3;    1;    25-;    (HL-60 cell);   

DOI  :  10.1016/0014-5793(89)80647-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carbonic anhydrase II (CA II) generates the H⁺ required for osteoclast-mediated bone resorption in humans. We have developed the human promyelocytic cell line HL-60 as a model system with which to study the osteoclast-specific expression of the CA II gene. Treatment of the cell line with 1,25-dihydroxyvitamin D₃ resulted in a dramatic de novo induction of CA II at both the protein and mRNA levels. CA II mRNA was also induced to a lesser extent by 12-O-tetradecanoyl phorbol 13-acetate. Treatment with dimethyl sulfoxide did not increase CA II mRNA. These findings indicate that the HL-60 cell line will be a useful model system to study the osteoclast-specific expression of the CA II gene.

【 授权许可】

Unknown