Reverse transcriptase‐polymerase chain reaction detects induction of cardiac‐like α myosin heavy chain mRNA in low frequency stimulated rabbit fast‐twitch muscle

FEBS Letters

Peuker, Heidemarie¹  Pette, Dirk¹ 

[1] Fakultät fur Biologie, Universitat Konstanz, Postfach 5560-M641, D-78434 Konstanz, Germany

关键词: Cardiac-like α myosin heavy chain;    Chronic low-frequency stimulation;    Rabbit skeletal muscle;    Reverse transcriptase-polymerase chain reaction;    ADM;    adductor magnus;    CLFS;    chronic low frequency stimulation;    DIA;    diaphragm;    EDL;    extensor digitorum longus;    GAS;    gastrocnemius;    MAM;    masseter;    MHC;    myosin heavy chain;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    SOL;    soleus;   

DOI  :  10.1016/0014-5793(95)00545-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using reverse transcriptase-polymerase chain reaction we quantified in rabbit skeletal muscles expression levels of the highly homologous cardiac α and β myosin heavy chain (αMHC, βMHC) mRNA isoforms. Masseter muscle displayed highest levels of a cardiac-like αMHC mRNA. This isoform was present at 20-fold lower amounts in slow soleus and at 200-fold lower levels in several fast-twitch muscles. Low-frequency stimulation periods exceeding 20 days drastically induced the αMHC mRNA in fast tibialis anterior. The αMHC mRNA was 140-fold elevated after 60 days when βMHC mRNA had increased 50-fold. Our results demonstrate the wide distribution of a cardiac-like αMHC mRNA in skeletal muscle and its marked induction during fast-to-slow transition as induced by low-frequency stimulation.

【 授权许可】

Unknown