Rapid and reversible changes in myosin heavy chain expression in response to increased neuromuscular activity of rat fast‐twitch muscle

FEBS Letters

Schneider, Susanne²  Mahdavi, Vijak¹  Pette, Dirk²  Kirschbaum, Bernhard J.²  Nadal-Ginard, Bernardo¹  Izumo, Seigo¹ 

[1] Laboratory of Molecular and Cellular Cardiology, Department of Cardiology, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA;Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750 Konstanz, FRG

关键词: Chronic low-frequency stimulation;    Myosin heavy chain mRNA isoform;    Fast-twitch muscle;   

DOI  :  10.1016/0014-5793(90)80976-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chronic 10 Hz stimulation of rat fast-twitch muscle induced rapid and reversible changes in the tissue levels offast myosin heavy chain (HC) mRNA isoforms. These changes consisted of a rapid decrease in HCIIb mRNA and a progressive increase in HCIIa mRNA. After 15 days, the HCIIb mRNA normally amounting to approximately 80%, had decreased to less than 5% of the sum of the two HC mRNA isoforms. HCIIb mRNA was again detectable one day after cessation of stimulation and progressively increased at the expense of HCIIa mRNA with ongoing recovery. These results point to a down-regulation of the HCIIb gene by the applied stimulus pattern which, conversely, enhances the expression of the HCIIa gene.

【 授权许可】

Unknown