Lutein associated with a transthyretin indicates carotenoid derivation and novel multiplicity of transthyretin ligands

FEBS Letters

Jörnval, Hans²  Pettersson, Tom³  Sjövall, Jan²  Bergman, Tomas²  Ernström, Ulf¹  Griffiths, William² 

[1] Department of Neuroscience, Karolinska Institutet, S-171 77 Stockholm, Sweden;Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden;Department of Clinical Chemistry, Karolinska Institutet, Danderyd Hospital S-182 88 Danderyd, Sweden

关键词: Lutein;    Carotenoid binding;    Transthyretin;    Yellow component;    Mass spectrometry;   

DOI  :  10.1016/0014-5793(95)00389-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transthyretins isolated from different species bind hydrophobic compounds and are often obtained in a yellow form. Such a transthyretin from chicken serum was purified by chromatography using Sepharose-coupled human retinol-binding protein. The yellow chromophore was extracted with methanol and purified by reverse phase HPLC followed by normal-phase chromatography on a nitrile column. Ultraviolet-visible absorbance and mass spectrometry identified the yellow compound as lutein, i.e. xanthophyll, (all-trans)-β,ϵ-carotene-3,3′-diol, estimated to constitute 10–30% of associated colourless compounds. These components are different from the yellow component isolated from human transthyretin and establish that carotenoid-derived pigments can be associated with transthyretins.

【 授权许可】

Unknown