A Factor That Prevents EDTA-Induced Cell-Growth Inhibition

Endocrine Journal

SHARAN PAUL¹  TAKAO SHINOZAWA¹  ISAMU SAKABE¹  TOSHIHIKO KADOYA²  TOMOHIRO MITSUMOTO¹ 

[1] Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University;Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd

关键词: Apoptosis inhibition;    Chick embryo brain;    Transthyretin;   

DOI  :  10.1507/endocrj.46.375

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(23)Cited-By(1)We previously reported the inhibition of cell-growth in Neuro-2A cells, mouse neuroblastoma, by Zn2+ chelation with EDTA. This paper describes the purification of a factor that prevents EDTA-induced cell-growth inhibition from chick embryo brain. The purified factor has a molecular mass of 16kDa on SDS-polyacrylamide gel electrophoresis under reducing conditions. This factor prevents the cell-growth inhibition in a dose-dependent manner and also binds thyroxine. Analysis of the N-terminal amino acid sequence revealed that 40 residues coincide with the sequence of chicken liver transthyretin.

【 授权许可】

Unknown