Cytochrome P450 CYP3A5 in the human anterior pituitary gland

FEBS Letters

Melvin, William T.¹  Burke, M.Danny³  Murray, Graeme I.²  Pritchard, Stuart¹ 

[1] Department of Molecular and Cell Biology, University of Aberdeen, Aberdeen, AB9 2ZD, UK;Department of Pathology, University of Aberdeen, Aberdeen, AB9 2ZD, UK;Department of Biomedical Sciences, University of Aberdeen, Aberdeen, AB9 2ZD, UK

关键词: Cytochrome P450;    Pituitary gland;    Somatotroph;    EPC;    diethyl pyrocarbonate;    P450;    cytochrome P450;    CYP 3A5;    the 5th member of the cytochrome P450 family 3A;    FITC;    fluorescein isothiocyanate;    RT-PCR;    reverse transcriptase polymerase chain reaction;    TBS;    0.05 M Tris-HCl-buffered saline pH 7.6;   

DOI  :  10.1016/0014-5793(95)00367-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytochromes P450 are a key group of enzymes involved in the metabolism of xenobiotics and several biologically active endogenous compounds. The expression of CYP3A5 has been identified by reverse transcriptase-polymerase chain reaction in human pituitary gland and shown by immunohistochemistry to be localized to growth hormone containing cells of the anterior pituitary gland. This is the first direct identification of an individual P450 subfamily in the pituitary gland and the presence of CYP3A in the pituitary gland may play a role in regulating growth hormone secretion.

【 授权许可】

Unknown