Abrupt pubertal elevation of DNase I gene expression in human pituitary glands of both sexes

FEBS Letters

Ueki, Misuzu³  Nakajima, Tamiko²  Takeshita, Haruo²  Kishi, Koichiro²  Yasuda, Toshihiro³  Iida, Reiko¹  Kaneko, Yasushi²  Mogi, Kouichi² 

[1] Department of Legal Medicine, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan;Department of Legal Medicine, Gunma University School of Medicine, Showa-machi, Maebashi, Gunma 371-8511, Japan;Department of Biology, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan

关键词: Age-dependent alteration;    Deoxyribonuclease I;    Gene expression;    Pituitary gland;    Puberty;    Human;    DAFO;    dried agarose film overlay;    DNase I;    deoxyribonuclease I;    IEF–PAGE;    isoelectric focusing in a thin layer of polyacrylamide gel;    LH-RH;    luteinizing hormone-releasing hormone;    SRED;    single radial enzyme diffusion;   

DOI  :  10.1016/S0014-5793(01)03216-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deoxyribonuclease I (DNase I) was confirmed to be expressed in the human pituitary gland, particularly the anterior lobe, at levels comparable to those in the pancreas. The DNase I activity and the amount of gene transcript present in the pituitary glands of individuals aged from 1 month to 89 years was significantly age-dependent, with an abrupt elevation after the neonatal and prepubertal periods irrespective of gender, followed by a gradual age-dependent decline in males and a marked reduction in females in their postreproductive period. This DNase I age dependence in the pituitary gland was not present in the pancreas and serum. These observations suggest that tissue-specific up-regulation of DNase I gene expression in the pituitary gland occur, possibly at the onset of puberty.

【 授权许可】

Unknown