Evidence for a distinct H7‐resistant form of protein kinase C in rat anterior pituitary gland

FEBS Letters

Clegg, Roger A.¹  MacEwan, David J.²  Ison, Angela J.³  Mitchell, Rory³  Connor, Kevin¹  Johnson, Melanie S.³ 

[1] Hannah Research Institute, Ayr, KA6 5HL, UK;Department of Biochemistry, S3-1, Institute of Biochemistry and Cell Biology, Syntex Discovery Research, 3401 Hillview Avenue, CA 94304, USA;MRC Brain Metabolism Unit, University Department of Pharmacology, 1 George Square, Edinburgh, EH8 9JZ, UK

关键词: Protein kinase C;    Pituitary gland;    Calcium dependence;   

DOI  :  10.1016/0014-5793(93)80221-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Inhibition of phorbol 12,13-dibutyrate-induced protein kinase C (PKC) activity from rat midbrain, anterior pituitary and a number of other tissues, as well as COS 7 cells, was studied in vitro. In anterior pituitary, Ca²⁺-independent activity was notably resistant to H7 but sensitive to staurosporine and Ro 31-8220. All Ca²⁺-dependent activity was sensitive to these three inhibitors. Mezerein and 1,2-dioctanoyl-sn-glycerol also activated this H7-insensitive PKC from anterior pituitary. The distribution of this activity, prominently expressed in pituitary and perhaps also lung, and its characteristic resistance to H7 but not other inhibitors, does not obviously correlate with that of any of the well-characterised PKCs, and may reflect either a novel or a modified isoform.

【 授权许可】

Unknown