Detection of histidine‐phospho‐proteins in animal tissues

FEBS Letters

Shaul, Yosef¹  Noiman, Silvia¹ 

[1] Department of Molecular Genetics and Virology, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Histidine kinase;    Nucleoside diphosphate kinase;    Phosphohistidine protein;    EDTA dependent phosphorylation;   

DOI  :  10.1016/0014-5793(95)00358-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this report we outline a protocol for rapid detection of histidine phosphoproteins in cellular crude extracts prepared from different tissues. The nature of the phosphorylated amino acid residues was confirmed by determination of their stability under different pH conditions and by direct phospho-amino acid analysis. Furthermore, DEPC treatment that can selectively modify the histidine residues blocks the phosphorylation. Interestingly, the phosphoprotein pattern detected under these conditions in four different tissues is very similar, suggesting that these proteins play important roles in biochemical pathways shared by many cells and tissues.

【 授权许可】

Unknown