Nucleoside diphosphate kinase from human erythrocytes: Purification, molecular mass and subunit structure

FEBS Letters

Presecan, Elena²  Lascu, Ioan¹  Vonica, Alin¹ 

[1] Department of Biochemistry, Medical and Pharmaceutical Institute, R-3400 Cluj-Napoca, Romania;Institute of Hygiene and Public Health, R-3400 Cluj-Napoca, Romania

关键词: Nucleoside diphosphate kinase;    Affinity chromatography;    Subunit structure;    (Human erythrocyte);    NDP kinase;    nucleoside diphosphate kinase (EC 2.7.4.6);    RBC;    red blood cells;    PAGE;    polyacrylamide gel electrophoresis;    BSA;    bovine serum albumin;    8-BrIDP;    8-bromoinosine 5′-diphosphate;   

DOI  :  10.1016/0014-5793(89)80811-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new procedure for the purification of nucleoside diphosphate kinase from human erythrocytes is described. The enzyme (105 kDa by gel filtration) is made up of two different kinds of subunits (19.0 and 20.5 kDa), both displaying enzymatic activity. The probable subunit structure of the enzyme is hexameric. The discrepancies related to earlier work are discussed.

【 授权许可】

Unknown