Na+‐independent Mg2+ efflux from Mg2+‐loaded human erythrocytes

FEBS Letters

Vormann, J.¹  Günther, T.¹ 

[1] Institute of Molecular Biology and Biochemistry, Free University of Berlin, Arnimallee 22, D-1000 Berlin 33, Germany

关键词: Mg2+ efflux;    Na+;    Cl−;    Acetamido-4′-isothiocyanatostilbene-2;    2′-disulfonic acid;    4-;    Amiloride;    (Human erythrocyte);    N+ o;    Cl− o;    extracellular concentrations of N+ or Cl−;    TCA;    trichloroacetic acid;    SITS;    4-acetamido-4′-isothiocyanatostilbene-2;    2′-disulfonic acid;    DIDS;    4;    4′-diisothiocyanatostilbene-2;    2′-disulfonic acid;   

DOI  :  10.1016/0014-5793(89)81329-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Net Mg²⁺ efflux from Mg²⁺-loaded human erythrocytes was maximal after reincubation in sucrose. Net Mg²⁺ efflux was not inhibited by furosemide or bumetanide and, therefore, was not performed by the (Na,K,Cl)- or (K,Cl)-cotransport system. A component of net Mg²⁺ efflux was inhibited by extracellular NaCl, KCl, LiCl, choline Cl and SITS, in analogy to the inhibition of net Cl⁻ and SITS. Therefore, it was concluded that net Mg²⁺ efflux is dependent on net Cl⁻ efflux for charge compensation. Cl⁻-dependent net Mg²⁺ efflux was inhibited by amiloride. Only 10% of the maximal net Mg²⁺ efflux may depend on extracellular Na⁺.

【 授权许可】

Unknown