Species‐specific Mn2+/Mg2+ antiport from Mg2+‐loaded erythrocytes

FEBS Letters

Vormann, J.²  Günther, T.²  Cragoe, E.J.¹ 

[1] Merck, Sharp and Dohme Research Laboratories, West Point, PA 19486, USA;Institute of Molecular Biology and Biochemistry, Free University of Berlin, Arnimallee 22,D-1000 Berlin 33, FRG

关键词: Mn2+ uptake;    Mg2+ efflux;    (Rat erythrocyte);    [Na+]0;    [Cl−]0;    [Mn2+]0;    extracellular concentration of Na+;    Cl−;    or Mn2+;    TCA;    trichloroacetic acid;    SITS;    4-acetamido-4' -isothiocyanatostilbene-2;    2' -disulfonic acid;   

DOI  :  10.1016/0014-5793(90)80633-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mg²⁺-loaded rat erythrocytes performed Mn⁺/Mg²⁺ antiport, which was nonspecifically stimulated by anions and cations. Mn²⁺/Mg²⁺ antiport was shown to operate via the Na⁺Mg²⁺ antiporter because extracellular Na⁺ and Mn²⁺ inhibited the intracellular uptake of each other's ions competitively. Furthermore, Mn²⁺/Mg²⁺ antiport and Na⁺/Mg²⁺ antiport were identically inhibited by various amiloride derivatives. Na⁺/Mg²⁺ antiport of chicken and human erythrocytes cannot perform Mn²⁺/Mg²⁺ antiport although chicken erythrocytes took up more Mn²⁺ than raterythrocytes.

【 授权许可】

Unknown