p56lyn catalyzes a reversible autophosphorylation reaction and a nucleoside diphosphate kinase reaction

FEBS Letters

Wang, Jerry H.¹  Litwin, Craig M.E.¹  Gendreau, Marthe¹ 

[1] MRC Group in Signal Transduction, Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada

关键词: Tyrosine kinase;    p56lyn autophosphorylation;    Nucleoside diphosphate kinase;   

DOI  :  10.1016/0014-5793(92)80788-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The reversible autophosphorylation of the pp60 family tyrosine kinase, p56^lyn has been characterized by a simple procedure that involves the examination of the enzyme catalyzed radioisotope exchange between ATP and ADP. The equilibrium constant of the reaction was determined to be 3.31 and corresponded to a standard free energy of hydrolysis of the phosphotyrosine bond in p56^lyn of −8.03 kcal/mol. GDP was capable of substituting for ADP as phosphate acceptor so that p56^lyn displayed a nucleoside diphosphate kinase activity.

【 授权许可】

Unknown