Phosphorylation of human plasminogen activators and plasminogen

FEBS Letters

Paracini, Francesco¹  De Petro, Giuseppina¹  Barlati, Sergio¹  Bona, Chiara¹  Tonelli, Mariella¹ 

[1] Division of Biology and Genetics, Department of Biomedical Sciences and Biotechnologies, University of Brescia, Via Valsabbina 19, 25123 Brescia, Italy

关键词: Human tissue-type plasminogen activator;    Urokinase-type plasminogen activator;    Human plasminogen;    Phosphorylation;    t-PA/u-PA;    tissue-type/urokinase-type plasminogen activator;    PG;    plasminogen;    SDS-PAGE;    sodium dodecyl sulphate-polyacrilamide gel electrophoresis;    HPLC;    high pressure liquid chromatography;    MoAb;    monoclonal antibodies;    AP;    alkaline phosphatase;    NBT;    nitroblue tetrazolium;    BCIP;    5-bromo-4-chloro-3-indolyl-phosphate;   

DOI  :  10.1016/0014-5793(95)00312-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plasminogen (PG), urokinase-type plasminogen activator (u-PA) and tissue-type PA (t-PA) are the main molecules involved in fibrinolysis and in many other physiological and pathological processes. In the present study we report that human t-PA, purified from human melanoma cells, and PG, purified from human plasma, both contain P-Tyr residues, as revealed by immunoblotting analyses with monoclonal anti-P-Tyr antibodies. In addition HPLC amino acid analysis of acid-hydrolyzed t-PA, PG and u-PA, shows that: (i)P-Ser and P-Tyr residues are present in t-PA; (ii)P-Thr and P-Tyr are present in PG; (iii) P-Ser, P-Thr and P-Tyr are present in u-PA. The utilization of monoclonal anti-P-Ser and anti-P-Thr antibodies in immunoblotting experiments has confirmed these data which indicate that phosphorylation is a common feature of PAs and of PG.

【 授权许可】

Unknown