A new member of the cytosolic O‐acetylserine(thiol)lyase gene family in Arabidopsis thaliana

FEBS Letters

Barroso, Consuelo¹  Vega, JoséM.¹  Gotor, Cecilia¹ 

[1] Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC y Universidad de Sevilla, 41080 Sevilla, Spain

关键词: O-Acetylserine(thiol)lyase;    Transcriptional regulation;    Sulfur deprivation;    Enzyme activation;    Arabidopsis thaliana;   

DOI  :  10.1016/0014-5793(95)00255-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA, Atcys-3A, encoding O-acetylserine-(thiol)lyase has been isolated from Arabidopsis thaliana. The deduced peptide sequence showed a high level of similarity with the bacterial counterpart, and a remarkable percentage of identity with other higher plant O-acetylserine(thiol)lyase genes. Sequence comparision and Southern blot analysis suggested that Atcys-3A was a new and different to the previously reported member of the cytosolic gene family in Arabidopsis. The Atcys-3A expression was activated by sulfur limitation, requiring a carbon and nitrogen source for maximal expression. A similar pattern of regulation was observed at the O-acetylserine(thiol)lyase activity level. Northern blot analysis also showed an organ-specific expression of Atcys-3A.

【 授权许可】

Unknown