FEBS Letters | |
Sequence specificity for removal of uracil from U·A pairs and U·G mismatches by uracil‐DNA glycosylase from Escherichia coli, and correlation with mutational hotspots | |
Krokan, Hans E.1  Nilsen, Hilde1  Eftedal, Ingrid1  Yazdankhah, Siamal Pour1  | |
[1] UNIGEN Center for Molecular Biology, University of Trondheim, N-7007 Trondheim, Norway | |
关键词: Uracil-DNA glycosylase; Sequence specificity; Mutational hotspot; | |
DOI : 10.1016/0014-5793(95)00244-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The rate of removal of uracil from different positions in double-stranded DNA by uracil-DNA glycosylase from Escherichia coli varied more than 15-fold. Consensus sequences for good and poor removal were 5′-(A/T)UA(A/T)-3′ and 5′-(G/C)U(T/G/C)-3′, respectively. In general, the sequence context surrounding U was more important for the rate of removal than whether U was present in U·A pairs or U·G mispairs. Rates of removal of U from sites of amber mutations in the lacI gene, where mutation frequencies and deamination rates were known, indicated that the observed variation in removal is biologically significant.
【 授权许可】
Unknown
【 预 览 】
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