Sequence specificity for removal of uracil from U·A pairs and U·G mismatches by uracil‐DNA glycosylase from Escherichia coli, and correlation with mutational hotspots

FEBS Letters

Krokan, Hans E.¹  Nilsen, Hilde¹  Eftedal, Ingrid¹  Yazdankhah, Siamal Pour¹ 

[1] UNIGEN Center for Molecular Biology, University of Trondheim, N-7007 Trondheim, Norway

关键词: Uracil-DNA glycosylase;    Sequence specificity;    Mutational hotspot;   

DOI  :  10.1016/0014-5793(95)00244-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rate of removal of uracil from different positions in double-stranded DNA by uracil-DNA glycosylase from Escherichia coli varied more than 15-fold. Consensus sequences for good and poor removal were 5′-(A/T)UA(A/T)-3′ and 5′-(G/C)U(T/G/C)-3′, respectively. In general, the sequence context surrounding U was more important for the rate of removal than whether U was present in U·A pairs or U·G mispairs. Rates of removal of U from sites of amber mutations in the lacI gene, where mutation frequencies and deamination rates were known, indicated that the observed variation in removal is biologically significant.

【 授权许可】

Unknown