Structure of the gene for human uracil—DNA glycosylase and analysis of the promoter function

FEBS Letters

Lund, Henning¹  Krokan, Hans E.¹  Skorpen, Frank¹  Haug, Terje¹ 

[1] UNIGEN Center for Molecular Biology, University of Trondheim, N-7005 Trondheim, Norway

关键词: Uracil-DNA glycosylase;    Gene expression;    Promoter function;   

DOI  :  10.1016/0014-5793(94)01042-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene for human uracil-DNA glycosylase (UNG) contains 4 exons and has an approximate size of 13 kb. The promoter is very GC rich and lacks a TATA box. Nested deletions of the promoter demonstrated that two SPI elements and a putative c-MYC element proximal to the transcription initiation region were sufficient to support some 27% of the promoter activity, while a clone that in addition contained the elements E2F/SP1/CCAAT increased expression to almost 90% of the full-length construct. A region upstream of these elements appears to exert a negative control function.

【 授权许可】

Unknown