期刊论文详细信息
FEBS Letters | |
Structure of the gene for human uracil—DNA glycosylase and analysis of the promoter function | |
Lund, Henning1  Krokan, Hans E.1  Skorpen, Frank1  Haug, Terje1  | |
[1] UNIGEN Center for Molecular Biology, University of Trondheim, N-7005 Trondheim, Norway | |
关键词: Uracil-DNA glycosylase; Gene expression; Promoter function; | |
DOI : 10.1016/0014-5793(94)01042-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The gene for human uracil-DNA glycosylase (UNG) contains 4 exons and has an approximate size of 13 kb. The promoter is very GC rich and lacks a TATA box. Nested deletions of the promoter demonstrated that two SPI elements and a putative c-MYC element proximal to the transcription initiation region were sufficient to support some 27% of the promoter activity, while a clone that in addition contained the elements E2F/SP1/CCAAT increased expression to almost 90% of the full-length construct. A region upstream of these elements appears to exert a negative control function.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020300181ZK.pdf | 474KB | download |