An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Malaria Journal

Methodology

Eleanor H Wong¹  Paul Horrocks²  Sandra Hasenkamp² 

[1] Infection and Immunity and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, 120 University Place, G12 8QQ, Glasgow, UK;Institute for Science and Technology in Medicine, Keele University, ST5 5BG, Staffordshire, UK;

关键词: Luciferase;    Single-step lysis;    Stage-specific;    Promoter function;    pcna;   

DOI  :  10.1186/1475-2875-11-42

 received in 2011-12-16, accepted in 2012-02-10,  发布年份 2012

来源: Springer

【 摘 要 】

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

【 授权许可】

Unknown   
© Hasenkamp et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.