Novel modification of ceramide: rat glioma ganglioside GM3 having 3‐O‐acetylated sphingenine

FEBS Letters

Inaba, Kotomi²  Suetake, Keiji¹  Gasa, Shinsei²  Ibayashi, Yukihiro¹  Chiba, Masahiko¹  Tsuchihashi, Keiko²  Hashi, Kazuo¹ 

[1] Department of Neurological Surgery, School of Medicine, Sapporo Medical University, Sapporo, Japan;Chemistry, School of Medicine, Sapporo Medical University, Chuo-ku, S1 W17, Sapporo 060, Japan

关键词: Ganglioside;    Glioma;    O-Acetyl sphingenine;    O-Acetyl ceramide;    O-Acetyl GM3;    Rat;    GSL;    glycosphingolipid;    Ggl;    ganglioside;    Ac;    acetyl;    Cer;    ceramide containing 4-sphingenine;    GM3;    N-acetylneuraminyl-lactosylceramide;    2D COSY;    two-dimensional chemical shift-correlated spectroscopy;    FAB-MS;    fast atom bombardment-mass spectrometry;    TLC;    thin-layer chromatography;    CMW;    chloroform/methanol/water;   

DOI  :  10.1016/0014-5793(95)00182-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel O-acetylated GM3 containing 3-O-acetyl 4-sphingenine was isolated with one having a non-acetylated base from transplanted rat glioma tissue. The presence and position of the acetyl group were estimated by one- and two-dimensional proton nuclear magnetic resonance, and fast atom bombardmentmass spectrometries. In addition, the O-acetyl showed higher immunological activity toward anti-melanoma antibody in the presence of non-acetylated GM3 in complement-dependent liposome lysis than did non-acetylated or acetylated GM3 alone in the liposome, suggesting enhancement of immunological reactivity of the intact tumor cells by a small amount of O-acetyl GM3.

【 授权许可】

Unknown