Action of influenza virus neuraminidase on gangliosides

FEBS Letters

Bergelson, L.D.²  Prokazova, N.V.²  Bukrinskaya, A.G.¹  Reshetov, P.D.²  Shaposhnikova, J.I.²  Slepushkin, V.A.¹  Zhigis, L.S.² 

[1] Ivanovsky Institute of Virology, USSR Academy of Medical Sciences, Gamaleya Str., 16, 123098, Moscow GSP-V-437, 117871, USSR;Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Miklukho-Maklaya str., 16/10, Moscow, GSP-V-437, 117871, USSR

关键词: Influenza virus receptor;    Viral neuraminidase;    Ganglioside;    NA;    neuraminidase;    PPNA;    partially purified neuraminidase;    HA;    haemagglutinin;    IV;    intact influenza A virus;    SRID;    single radial immunodiffusion;    ganglioside abbreviations according to Svennerholm [1];   

DOI  :  10.1016/0014-5793(85)80314-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The action of partly purified neuraminidase (NA) of influenza A virus, a mixture of detergent solubilized NA and haemagglutinin (HA) and of intact virions on gangliosides G_Tlb, g_dla, G_Dlb, g_ml was studied. The viral NA transformed g_tlb mainly into G_DlbWith formation of only minor amounts of G_Ml. HA was found to inhibit the hydrolysis activity of viral NA. At the same time viral NA transformed G_Dla quantitatively into g_ml which was not hydrolyzed by the enzyme. These results suggest that the function of NA is to transfer the ‘primary’ receptor (such as G_Tlb) into the proper carbohydrate sequence (G_Dlb-like) which is proposed to serve as the minimal structure required for influenza virus reception.

【 授权许可】

Unknown