Generation of inositol phosphates, cytosolic Ca2+ and secretion of noradrenaline in PC12 cells treated with glutamate

FEBS Letters

Nomura, Yasuyuki¹  Murayama, Toshihiko¹  Kurozumi, Kouichi¹ 

[1] Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: Glutamate;    Phosphoinositide;    Cytosolic Ca2+;    PC12 cell;    NMDA;    N-methyl-D-aspartate;    PIs;    phosphoinositides;    NA;    noradrenaline;    PMA;    phorbol 12-myristate 13-acetate;    APV;    DL-2-amino-5-phosphonovalerate;    Fura-2/AM;    fura-2/acetoxymethylester;    DMEM;    Dulbecco's modified Eagle's medium;    IP3;    inositol trisphosphate;    [Ca2+]i;    the cytosolic free Ca2+ concentration;   

DOI  :  10.1016/0014-5793(90)81274-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glutamate transiently stimulated rat pheochromocytoma PC12 cells and caused an inositol trisphosphate formation and an increase in levels of Ca⁺ in the cytosol. The rank order of potency of glutamate > N-methyl-D-aspartate (NMDA) > kainate = quisqualate is characteristic of an interaction with NMDA receptors. The effect of glutamate on inositol trisphosphate formation disappeared in a low Mg²⁺ buffer and was not blocked by DL-2-amino-5-phosphonovalerate, an antagonist for NMDA receptors coupled to ion channels. Although glutamate failed to stimulate noradrenaline secretion, glutamate enhanced the effect of bradykinin, but not of Ca ionophore A23187, or KC1. These results suggest the existence of metabotropic glutamate receptors, different from previously reported receptors, in PC12 cells.

【 授权许可】

Unknown