Astaxanthin protects PC12 cells from glutamate-induced neurotoxicity through multiple signaling pathways

Journal of Functional Foods

Lijuan Zhang¹  Xiangzhao Mao²  Yiran Zhang³  Wei Wang³  Cui Hao³ 

[1] Corresponding author. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Tel.: [phone];College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China;

关键词: Astaxanthin;    Neuroprotective;    PC12 cell;    Glutamate;    Oxidative stress;    Nuclear factor-kappa B;   

DOI  :  

来源: DOAJ

【 摘 要 】

Astaxanthin, a carotenoid extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have neuroprotective effect. In this study, the neuroprotective effects and mechanisms of astaxanthin against glutamate-induced neurotoxicity were explored. The results showed that pretreatment with astaxanthin inhibited neuronal PC12 cell death induced by L-glutamate and reduced the production of reactive oxygen species (ROS) significantly. Astaxanthin prevented glutamate-induced apoptosis by depressing the elevation of Bax/Bcl-2 ratio, caspase-3 activation and Ca2+ influx. Moreover, the loss of mitochondrial membrane potential (MMP) as well as the activation of ROS associated NF-κB and MAPK pathways were also inhibited upon treatment with astaxanthin. Therefore, the neuroprotective effects reflected the ability of astaxanthin in the regulation of multiple signaling pathways that protect mitochondria as well as cellular damage induced by L-glutamate. Thus, astaxanthin has the potential to be used as a prophylactic or remediation agent against neuronal disorders.

【 授权许可】

Unknown