On the origin of a sustained increase in cytosolic Ca2+ concentration after a toxic glutamate treatment of the nerve cell culture

FEBS Letters

Khodorov, B.³  Golovina, V.³  Andreeva, N.¹  Khaspekov, L.¹  Victorov, I.¹  Pinelis, V.²  Uvarova, T.²  Fajuk, D.³ 

[1]Brain Research Institute, Russian Academy of Medical Sciences, Moscow, Russian Federation

[2]Institute of Pediatrics, Russian Academy of Medical Sciences, Moscow, Russian Federation

[3]Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russian Federation

关键词: Glutamate;    Neurotoxicity;    Cytosolic Ca2+;    Na+/Ca2+ exchange;    Nerve cell culture;    [Ca2+]i;    cytosolic free calcium;    [Ca2+]o;    extracellular free calcium;    pHi;    cytosolic pH;    Fura-2/AM;    acetoxymethyl ester of fura-2;    HEPES;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    EGTA;    ethylene glycol bis(β-aminoethylether)-N;    N'-tetraacetic acid;    NMDA;    N-methyl-d-aspartate;    TMA;    tetramethylammonium;    DCB;    dichlorbenzamil;    BS;    buffered salt solution;    CS;    control solution;   

DOI  :  10.1016/0014-5793(93)80132-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A sustained increase of cytosolic Ca²⁺ concentration, [Ca²⁺]_i, (Ca²⁺ plateau) was induced by a 15-min treatment with 50 μM glutamate of cultured cerebellar granule cells and hippocampal neurons in a Mg²⁺-free solution. Plateau proved to be insensitive to inhibition of Na⁺ _o/Ca²⁺ _i exchange caused by removal external Na⁺ in the post-glutamate period. A ~ 10⁵-fold reduction of [Ca²⁺]_o (from 1.5 mM to 20 nM) in the post-glutamate period caused in most cells only a slow and small decrease in [Ca²⁺]_i, although the same low-Ca²⁺ trial before glutamate treatment caused in hippocampal cells very quick blockade of spontaneous [Ca²⁺]_i oscillation and a decrease in the basal [Ca²⁺]_i. The results suggest that the Ca²⁺ plateau is due to a suppression of the Ca²⁺ extrusion from the cell (in particular via Na⁺/Ca²⁺ exchange) rather than from a persistent increase in Ca²⁺ permeability of neuronal membrane.

【 授权许可】

Unknown