Activation of phosphatidylinositol synthesis by different agonists in a primary culture of smooth muscle cells grown on collagen microcarriers

FEBS Letters

Berta, Philippe³  Travo, Pierre¹  Bockaert, Joël²  Sladeczek, Fritz²  Haiech, Jacques³ 

[1] Laboratoire de Pharmacodynamique CNRS VA 600, Université Louis Pasteur BP 10, 67400 Illkirch Cedex, France;Centre CNRS-INSERM de Pharmacologie-Endocrinologie, BP 5055, 34033 Montpellier Cedex, France;Centre de Recherches de Biochimie macromoléculaire LP8402 du CNRS et INSERM U249, BP 5051, France

关键词: (Aortic myocyte) Cell culture Muscle contraction Agonist Inositide metabolism;    All;    angiotensin II;    AVP;    (Arg)vasopressin;    NA;    noradrenaline;    5-HT;    5-hydroxytryptamine;    IP;    myo-inositol phosphate;    IP3;    myo-inositol 1;    4;    5-trisphosphate;    PBS;    phosphate-buffered saline;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    d(CH2)5-AVP;    [1-β-mercapto-β-(β' -cyclopentamethylene-propionic acid)-8-arginine] vasopressin;   

DOI  :  10.1016/0014-5793(86)80504-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Regulation of inositol phosphate synthesis was examined in a primary culture of vascular smooth muscle cells grown on collagen-coated microcarriers. In the presence of Lid (10 mM), four agonists [serotonin, angiotensin, (arginine) vasopressin and noradrenaline] were found to stimulate the formation of inositol phosphates in a dose-dependent manner. All agonists were found to have identical and additive effects on the time course of inositol phosphate formation. Therefore, our primary cell culture technique was proved to give smooth muscle cells suitable for the study of modulation of phosphoinositide metabolism in response to physiological effectors.

【 授权许可】

Unknown