Fucosyl gangliosides of PC12 pheochromocytoma cells

FEBS Letters

Mazzulla, M.¹  Greene, L.A.¹  Margolis, R.K.¹  Margolis, R.U.¹ 

[1] Department of Pharmacology, New York University Medical Center, New York, NY 10016, USA

关键词: Ganglioside;    PC12 pheochromocytoma cell;    Glycolipid Fucose;   

DOI  :  10.1016/0014-5793(84)81153-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three monosialogangliosides are highly labeled when PC12 pheochromocytoma cells are cultured in the presence of L-[³H] fucose, and two additional monosialogangliosides are labeled to a lesser extent. In contrast, neither of the two disialogangliosides of PC12 cells contains fucose residues. Removal of sialic acid and fucose by formic acid hydrolysis demonstrated the presence of 3 major ‘core’ structures in the monosialogangliosides, and a single asialo derivative of the disialogangliosides which has the same chromatographic mobility as one of the monosialoganglioside hydrolysis products. None of the major formic acid hydrolysis products of the PC12 cell gangliosides corresponds to asialo-GM₁, supporting our previous conclusion that PC12 cells do not contain significant amounts of brain-type gangliosides.

【 授权许可】

Unknown