The membrane distal half of gp130 is responsible for the formation of a ternary complex with IL‐6 and the IL‐6 receptor

FEBS Letters

Schmitz-Van de Leur, Hildegard¹  Horsten, Ursula¹  Heinrich, Peter C.¹  Müllberg, Jürgen³  Rose-John, Stefan² 

[1] Institut für Biochemie, RWTH Aachen, Pauwelsstraße 30, 52057 Aachen, Germany;I. Medizinische Klinik der Johannes Gutenberg-Universität Mainz, Abteilung Pathophysiologie, Obere Zahlbacher Straße 63, 55101 Mainz, Germany;Immunex Corp., University Street, Seattle, WA 98101, USA

关键词: gp130;    Structure-function analysis;    Cytokine receptor domain;    Interleukin-6;    CNTF;    ciliary neurotrophic factor;    gp130;    glycoprotein 130;    GM-CSF;    granulocyte macrophage colony stimulating factor;    IgG;    immunoglobulin G;    IL;    interleukin;    LIF;    leukemia inhibitory factor;    OM;    oncostatin M;   

DOI  :  10.1016/0014-5793(95)00053-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gp130 is the signal transducing subunit of the interleukin-6 receptor. Signaling is initiated by the complex formation of gp130 with IL-6 bound to the IL-6 receptor (IL-6R). We have subdivided the extracellular domain of gp130 in two parts and expressed the mutant proteins as soluble IgG fusion proteins in COS-7 cells. By studying the formation of the ternary complex we show that the membrane distal half of gp130 which contains a cytokine receptor domain is responsible for the interaction with the IL-6/IL-6R complex. Interestingly this is the same region which is believed to be involved in specific recognition of the related cytokines LIF, OM, and probably also of CNTF and IL-11.

【 授权许可】

Unknown