FEBS Letters | |
Isolation and identification of a μ‐calpain‐protein kinase Cα complex in skeletal muscle | |
Verret, Catherine1  Savart, Michel1  Touyarot, Katia1  Dutaud, Dominique1  Elamrani, Najat1  Ducastaing, André1  | |
[1] ISTAB, Université Bordeaux 1, Avenue des Facultés, 33405 Talence Cedex, France | |
关键词: Protein kinase C; Calpain; Skeletal muscle; | |
DOI : 10.1016/0014-5793(95)00014-Z | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A μ-calpain-PKC complex was isolated from rabbit skeletal muscle by ultracentrifugation and by anion-exchange chromatography. The PKC associated to μ-calpain was stimulated by calcium, phosphatidylserine and diacylglycerol, and corresponds to a conventional PKC (cPKC). This complex presents an apparent molecular mass close to 190 kDa and is composed of one μ-calpain molecule and of one cPKC molecule. Using monoclonal antibodies specific for the different cPKC isoforms, the isoenzyme associated to μ-calpain was identified as cPKCα. Immunofluorescence staining reveals a co-localization of μ-calpain and cPKCα on the muscle fibre plasma membranes.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020300650ZK.pdf | 489KB | download |