Isolation and identification of a μ‐calpain‐protein kinase Cα complex in skeletal muscle

FEBS Letters

Verret, Catherine¹  Savart, Michel¹  Touyarot, Katia¹  Dutaud, Dominique¹  Elamrani, Najat¹  Ducastaing, André¹ 

[1] ISTAB, Université Bordeaux 1, Avenue des Facultés, 33405 Talence Cedex, France

关键词: Protein kinase C;    Calpain;    Skeletal muscle;   

DOI  :  10.1016/0014-5793(95)00014-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A μ-calpain-PKC complex was isolated from rabbit skeletal muscle by ultracentrifugation and by anion-exchange chromatography. The PKC associated to μ-calpain was stimulated by calcium, phosphatidylserine and diacylglycerol, and corresponds to a conventional PKC (cPKC). This complex presents an apparent molecular mass close to 190 kDa and is composed of one μ-calpain molecule and of one cPKC molecule. Using monoclonal antibodies specific for the different cPKC isoforms, the isoenzyme associated to μ-calpain was identified as cPKCα. Immunofluorescence staining reveals a co-localization of μ-calpain and cPKCα on the muscle fibre plasma membranes.

【 授权许可】

Unknown