An SH3 domain is required for the mitogenic activity of microinjected phospholipase C‐γ1

FEBS Letters

Davis, Lenora¹  Wegrzyn, Ron E.¹  Huang, Pearl S.¹  Oliff, Allen¹  Huber, Hans¹  Heimbrook, David C.¹  Goodhart, Paula J.¹ 

[1] Department of Cancer Research, Building WP16-3, Merck Research Laboratories, West Point, PA 19486, USA

关键词: Phospholipase C;    SH2;    SH3;    Mitogenic stimulation;    Microinjection;   

DOI  :  10.1016/0014-5793(94)01453-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phospholipase activity is elevated in dividing cells. In response to growth factor stimulation, phospholipase C-γ (PLC-γ) binds to activated tyrosine kinase receptors via SH2 binding domains, resulting in phosphorylation of PLC-γ and activation of its enzyme activity. These observations suggest that PLC-γ participates in the signal transduction pathway employed by growth factors to promote mitogenesis. Consistent with this hypothesis, microinjection of purified bovine PLC-γ into quiescent fibroblasts has been previously reported to initiate a mitogenic response [Smith et al. (1989) Proc. Natl. Acad. Sci. 86, 3659]. We have reproduced this result using recombinant rat PLC-γ protein. Surprisingly, however, a catalytically inactive mutant of PLC-γ, H335Q, also elicited a full mitogenic response. The capacity to induce mitogenesis by microinjection of PLC-γ was mapped to the ‘Z’ domain of the protein, which contains PLC-γ's SH2 and SH3 motifs. Inactivation of the phosphorylated tyrosine binding properties of both SH2 domains had no effect on the mitogenic activity of the Z-domain peptide. However, deletion of the SH3 domain resulted in a complete loss of activity. These results suggest that PLC-γ's mitogenic properties do not require the enzyme's phospholipase activity, but are instead mediated by a novel pathway for mitogenic stimulation which is dependent upon an intact SH3 domain.

【 授权许可】

Unknown