Distinct roles of the Src family kinases, SRC‐1 and KIN‐22, that are negatively regulated by CSK‐1 in C. elegans

FEBS Letters

Okada, Masato¹  Hirose, Takashi¹  Ohshima, Yasumi²  Koga, Makoto² 

[1] Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan;Department of Biology, Faculty of Sciences, Kyushu University Graduate School, Fukuoka 812-8581, Japan

关键词: csk-1;    src-1;    kin-22;    Tyrosine kinase;    Caenorhabditis elegans;    SFK;    Src family kinase;    PTK;    protein tyrosine kinase;    Csk;    C-terminal Src kinase;    SH2;    Src homology 2;    SH3;    Src homology 3;    SMART;    simple module architecture research tool;    DIC;    differential interference contrast;   

DOI  :  10.1016/S0014-5793(02)03819-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To elucidate the primitive roles of the Src family kinases (SFKs), here we characterized Caenorhabditis elegans orthologues of SFKs (src-1 and kin-22) and their regulator kinase Csk (csk-1). SRC-1 and KIN-22 possess the C-terminal regulatory tyrosines characteristic of SFKs, and their activities are negatively regulated by CSK-1 in a yeast expression system. The src-1 and csk-1 genes are co-expressed in some head neurons, the anchor cell and the tail region, while kin-22 and csk-1 genes are co-expressed in pharyngeal muscles and tail region. Expression of KIN-22 induced morphological defects in the pharynx, whereas expression of SRC-1 did not show any overt phenotype in adult. RNA interference of src-1, but not that of kin-22, caused a developmental arrest in early development. These results suggest that SRC-1 and KIN-22 play distinct roles under the control of CSK-1.

【 授权许可】

Unknown