Characterization of a protein tyrosine phosphatase (RIP) expressed at a very early stage of differentiation in both mouse erythroleukemia and embryonal carcinoma cells

FEBS Letters

Mukouyama, Yousuke¹  Oishi, Michio¹  Nomura, Nobuo²  Chida, Dai¹  Kume, Tsutomu¹  Thomas, Matthew L.³  Tabata, Satoshi²  Watanabe, Toshio¹ 

[1] Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo, Tokyo 113, Japan;Kazusa DNA Research Institute, Kisarazu, Chiba 292, Japan;Howard Hughes Medical Institute and Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA

关键词: Protein tyrosine phosphatase;    Erythroleukemia cell;    Chromosomal mapping;    Differentiation;   

DOI  :  10.1016/0014-5793(94)01432-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

From our previous studies, several protein tyrosine phosphatases (PTPase) are implicated in the early events leading to in vitro differentiation of both mouse erythroleukemia (MEL) and embryonal carcinoma (F9) cells. Among the PTPases, recent experiments suggest that a new PTPase (RIP) plays a critical role in differentiation processes, particularly at their early stages. We isolated cDNA clones for RIP from a RNA preparation isolated from differentiating MEL cells, and determined the total 7932 bp base sequence for RIP cDNA. The cDNA codes for a putative 269.8 kDa (2450 amino acids) protein with a PTPase catalytic domain. We have demonstrated that the transcripts exist in multiple forms, and among mouse tissues they were found predominantly in kidney and, to a lesser extent, in lung, heart, brain and testis. The RIP gene was mapped between D5Mit90 and D5Mit25 on mouse chromosome 5.

【 授权许可】

Unknown