Pervanadate elicits proliferation and mediates activation of mitogen‐activated protein (MAP) kinase in the nucleus

FEBS Letters

Krady, Marie-Marthe¹  Rogue, Patrick¹  Malviya, Anant N¹  Freyermuth, Solange¹ 

[1] Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, UPR 416 du CNRS, Centre de Neurochimie, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France

关键词: Protein tyrosine phosphatase;    Protein tyrosine kinase;    Pervanadate;    Mitogen-activated protein kinase;    TPA;    Proliferation;    Differentiation;    C3H10T1/2 mouse fibroblast;   

DOI  :  10.1016/S0014-5793(97)00821-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

There is growing evidence for the role of protein tyrosine phosphatases in controlling such fundamental cellular processes as growth and differentiation. Pervanadate is a potent inhibitor of protein tyrosine phosphatase which has been observed here to induce proliferation in C3H10T1/2 mouse fibroblasts. Pervanadate also translocated/activated p42/44 mitogen-activated protein (MAP) kinase to the cell nucleus. An almost similar pattern of nuclear p42/44 MAP kinase stimulation is seen with TPA. On the other hand, TPA treatment results in a rapid activation of cytosolic MAP kinase which declines with time. Thus pervanadate appears as a very useful tool for studying tyrosine phosphorylation.

【 授权许可】

Unknown