Pervanadate‐triggered MAP kinase activation and cell proliferation are not sensitive to PD 98059

FEBS Letters

Krady, Marie-Marthe²  Dupont, Jean-Luc¹  Malviya, Anant N.² 

[1] Laboratoire de Neurobiologie Cellulaire, CNRS UPR 9009, 5 rue Blaise Pascal, 67084 Strasbourg, France;Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, CNRS UPR 416, 5 rue Blaise Pascal, 67084 Strasbourg, France

关键词: Mitogen-activated protein kinase;    Cell proliferation;    PD 98059;    Tyrosine phosphatase;    Pervanadate;   

DOI  :  10.1016/S0014-5793(98)00989-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A tight and stable complex with corresponding protein kinases and phosphatases establishes coupling between activators and inactivators. One such example is emerging from the studies of the Ras-dependent MAP kinase cascade signaling pathway. Pervanadate, a potent inhibitor of protein tyrosine phosphatase, stimulates MAP kinase and elicits cell proliferation in cultured mouse fibroblasts which is insensitive to PD 98059, the major inhibitor of upstream MEK, whereas serum- or TPA- triggered proliferation is sensitive to PD 98059. It is suggested that imbalanced coordination between protein kinase and protein phosphatase determines the cellular responses such as cell proliferation. The PD 98059-insensitive cell proliferation upon protein tyrosine phosphatase inhibition is attributed to a MEK bypass pathway.

【 授权许可】

Unknown