Role of MAP kinase cascades in inducing arginine transporters and nitric oxide synthetase in RAW264 macrophages

FEBS Letters

Caivano, Matilde¹ 

[1] MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, UK

关键词: Lipopolysaccharide;    Inflammation;    Arginine transport;    Nitric oxide synthetase;    PD 98059;    SB 203580;   

DOI  :  10.1016/S0014-5793(98)00578-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bacterial lipopolysaccharide (LPS) in the presence of interferon gamma (IFNγ) stimulates the synthesis of the cationic amino acid transporter 2B (CAT-2B) and inducible nitric oxide synthetase (iNOS) in RAW264 macrophages, which are thought to underlie the increased rate of arginine uptake into these cells and its conversion to nitric oxide, respectively. Here I demonstrate that the LPS- and IFNγ-induced increase in arginine uptake into RAW264 cells is partially suppressed in the presence of PD 98059, partially suppressed in the presence of SB 203580, and completely inhibited by both drugs. In contrast, the LPS- and IFNγ-induced synthesis of CAT-2B mRNA and iNOS protein is unaffected by PD 98059 and SB 203580. The results indicate that the MAPK/ERK and SAPK2/p38 cascades are both rate-limiting for LPS- and IFNγ-stimulated arginine uptake, but not for iNOS synthesis. They also suggest that PD 98059 and SB 203580 suppress CAT-2B synthesis at a post-transcriptional level.

【 授权许可】

Unknown