Induction of apoptotic DNA fragmentation and cell death by natural ceramide

FEBS Letters

Ji, Li²  Hirabayashi, Yoshio²  Uematsu, Shogo¹  Zhang, Gu²  Akahori, Yukio¹ 

[1] Laboratory of Physical Chemistry, School of Pharmaceutical Science, University of Shizuoka, 52-1 Yada, Shizuoka-shi 422, Japan;Laboratory for Glyco Cell Biology, Frontier Research Program, The Institute of Chemical and Physical Research (RIKEN), 2-1 Hirosawa, Wako-shi, Saitama 351-01, Japan

关键词: Pregrammed DNA fragmentation;    Apoptosis;    TNFα;    Ceramide;    Sphingomyelinase;    DMEM;    Dulbecco's modified Eagle's medium;    F12;    Ham's F12 medium;    SM;    sphingomyelin;    Cer;    ceramide;    Sph;    sphingosine;    SMase;    sphingomyelinase;    TNFα;    tumor necrosis factor α;    C2-Cer;    N-acetylsphingosine;    DAG;    diacylglycerol;    C6-NBD-Cer;    PMA;    4β-phorbo 12-myristate 13-acetate;    EDC;    1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride;   

DOI  :  10.1016/0014-5793(94)01428-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have found that a solvent mixture of ethanol and dodecane was able to disperse natural ceramide (Cer) into aqueous solution. In U937 cells, addition of natural Cer to medium caused a characteristic pattern of DNA fragmentation, which is indistinguishable from that caused by TNFα. The effective concentration of Cer is as low as 25 nM. The present study provides direct evidence that natural Cer functions as a second messenger mediating TNFα-induced DNA fragmentation. The use of this solvent to deliver hydrophobic natural Cer to cells will contribute to the elucidation of the biological function of Cer.

【 授权许可】

Unknown