Kinetic studies on the formation of acridine‐linked DNA triple helices

FEBS Letters

Fox, Keith R.¹ 

[1] Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, UK

关键词: Triple helix;    Association kinetics;    DNase I footprinting;   

DOI  :  10.1016/0014-5793(94)01387-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used DNase I footprinting to measure the rate of intermolecular triple helix formation at the target sites A₆G₆ · C₆T₆ and G₆A₆ · T₆C₆ with the acridine-linked oligonucleotides Acr-T₅C₅, and Acr-C₅T₅ respectively. Under pseudo first-order reaction conditions we find that the reactions are slow, with half-lives of several minutes. The rates are dependent on the concentration of the third strand oligonucleotide and yield bimolecular association rate constants of 300 M⁻¹ · s⁻¹ for Acr-T₅C₅ binding to A₆G₆ · C₆T₆ and 2000 M⁻¹ · s⁻¹ for the interaction of Acr-C₅T₅ with G₆A₆ · T₆C₆.

【 授权许可】

Unknown