Spectroscopic evidence for an intramolecular RNA triple helix

FEBS Letters

Liquier, J.³  Huynh-Dinh, T.¹  Gouyette, C.¹  Guittet, E.²  Klinck, R.²  Taillandier, E.³ 

[1] Unité de Chimie Organique, URA CNRS 487 - Institut Pasteur, 75724 Paris, France;Institut de Chimie des Substances Naturelles, CNRS, 91198 Gif-sur-Yvette, France;Laboratoire CSSB, URA CNRS 1430 - Université Paris Nord, 93012 Bobigny, France

关键词: RNA structure;    NMR;    Triple helix;    Oligoribonucleotide synthesis;   

DOI  :  10.1016/0014-5793(94)01228-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 29-base RNA oligomer has been chemically synthesized and shown to form an intramolecular triple helix in solution at acidic pH. Assignment of the majority of the exchangeable proton NMR resonances demonstrated the Watson—Crick and Hoogsteen base pairings consistent with folding to form pyrimidine—purine—pyrimidine base triplets. FTIR spectroscopy provided independent evidence of base triplet formation, and indicated a predominately C3′-endo sugar pucker. UV absorption as a function of temperature suggested monophasic melting behaviour, which was confirmed by NMR of the imino protons.

【 授权许可】

Unknown