Triple helix formation at A8XA8·T8YT8

FEBS Letters

Chandler, Simon P.¹  Fox, Keith R.¹ 

[1] Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton, SO9 3TU, UK

关键词: Triple helix;    Sequence recognition;    Stringency;    DNase I footprinting;   

DOI  :  10.1016/0014-5793(93)80510-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the formation of DNA triple helices between the oligonucleotides T₈XT₈ (X = A,C,G,T) and DNA fragments containing the target sequences A₈XA₈ · T₈YT₈ (X = T,C,G; Y=A,G,C), by DNase I footprinting. We find that A₈GAg₈ · T₈CT₈ yields a footprint with T₈CT₈ and shows a weaker interaction with T₁₇ and T₈GT₈· A₈CA₈ · T₈GT₈ yields a footprint with T₁₇, and shows weaker interaction with T₈CT₈. A₈TA₈ · T₈AT₈ yields a footprint with T₈GT₈ and shows weaker interaction with T_17. Each of the successful complexes is characterised by enhanced DNase I cleavage at the 3' end of the purine strand of the target, as well as protection at the 5' end. We have been unable to form triplexes with third strands of the type A₈XA₈.

【 授权许可】

Unknown