Cleavage and sequence recognition of 2,6‐diaminopurine‐containing DNA by site‐specific endonucleases

FEBS Letters

Szekeres, Miklós¹  Matveyev, Andrey V.¹ 

[1] Institute of Plant Physiology, Biological Research Center, H-6701 Szeged, PO Box 521, Hungary

关键词: DNA;    Modified nucleotide;    Restriction endonuclease;    Sequence recognition;    (Bacteriophage);    DAP;    2;    6-diaminopurine;   

DOI  :  10.1016/0014-5793(87)80197-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The susceptibility of 2,6-diaminopurine (DAP)-containing bacteriophage DNA to several restriction and other endonucleases was examined. With the only exception of TaqI, these enzymes did not accept the modified base as a substitute for adenine. The phage DNA was extensively fragmented by the restriction endonucleases which recognize only G and C-containing sites. 5′-terminal analysis of the MspI and SmaI fragments revealed that d(DAP-T) basepairs can be mistaken by some enzymes for d(G-C) pairs.

【 授权许可】

Unknown